Correction: Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition

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There is an error in Fig 1. The image labeled ’No Cas9’ in Fig 1C is incorrect. Please see the correct Fig 1 here.

Fig 1. Analysis of five SaCas9 ortholog activities.

(A) Amino acid sequences of the SaCas9 ortholog PI domain are aligned. The residues that are important for PAM recognition are indicated at the top; the conserved residues among newly identified SaCas9 orthologs are shown in red; the names of newly identified Cas9s are shown in green. (B) Design of the GFP activation reporter construct. A target sequence (protospacer) containing a 7-bp random sequence is inserted between ATG and the GFP-coding sequence. The library DNA is stably integrated into HEK293T cells by lentivirus. (C) Transfection of SaCas9 orthologs induced GFP expression. Percentage of GFP-positive cells was shown. The cells without transfection of Cas9 were used as a negative control.

https://doi.org/10.1371/journal.pbio.3003036.g001

Citation: Wang S, Tao C, Mao H, Hou L, Wang Y, Qi T, et al. (2025) Correction: Identification of SaCas9 orthologs containing a conserved serine residue that determines simple NNGG PAM recognition. PLoS Biol 23(3): e3003036. https://doi.org/10.1371/journal.pbio.3003036

Published: March 13, 2025

Copyright: © 2025 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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